• Lonza’s MycoAlert® Mycoplasma Detection Assays are selective biochemical tests to detect Mycoplasma contamination in cell cultures.
Detection of Mycoplasma in cell cultures is performed as follows: • Viable mycoplasma in a test sample (cell culture supernatant) are lysed
• The released mycoplasmal enzymes react with the MycoAlert® Substrate
• The ATP is then transferred into a light signal via the luciferase enzyme in the MycoAlert® Reagent.
• Measuring the level of ATP in a sample both before (read A) and after the addition of the MycoAlert® Substrate (read B), a ratio can be determined that indicates the presence or absence of mycoplasma.
Upstream tips
• Lonza’s MycoAlert® Mycoplasma Detection Assays are selective biochemical tests to detect Mycoplasma contamination in cell cultures.
Protocol tips
Detection of Mycoplasma in cell cultures is performed as follows: • Viable mycoplasma in a test sample (cell culture supernatant) are lysed
• The released mycoplasmal enzymes react with the MycoAlert® Substrate
• The ATP is then transferred into a light signal via the luciferase enzyme in the MycoAlert® Reagent.
• Measuring the level of ATP in a sample both before (read A) and after the addition of the MycoAlert® Substrate (read B), a ratio can be determined that indicates the presence or absence of mycoplasma.
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