IMAGEN™ Parainfluenza Virus Group Kit using Direct Immunofluorescence Assay

Cell Culture Contamination Detection Kit Virus

Experiment

Cell Culture Contamination Detection Kit Virus

Product

IMAGEN™ Parainfluenza Virus Group Kit using Direct Immunofluorescence Assay from Thermo Fisher Scientific

Manufacturer

Thermo Fisher Scientific

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Tips Publication protocol Reviews Discussion Papers Manufacturer protocol Videos

Protocol tips

Upstream tips	Protocol tips	Downstream tips
• Kit detects and differentiates Parainfluenza virus types 1, 2 and 3 antigens directly in nasopharyngeal aspirates or in cell culture preparations. • Use IMAGEN Parainfluenza Kit as a rapid, sensitive, and specific method for detection and differentiation of Parainfluenza virus types 1, 2, and 3 antigens	• Specimens are incubated with the FITC conjugated reagents for 15 minutes. • Excess reagent is then removed by washing with phosphate-buffered saline (PBS). • The stained areas are mounted and viewed using epifluorescence illumination.	• Parainfluenza virus types 1, 2, or 3 are present then characteristic apple-green intracellular fluorescence is seen within infected cells, which contrasts with the red background staining of uninfected cells.

Upstream tips
• Kit detects and differentiates Parainfluenza virus types 1, 2 and 3 antigens directly in nasopharyngeal aspirates or in cell culture preparations. • Use IMAGEN Parainfluenza Kit as a rapid, sensitive, and specific method for detection and differentiation of Parainfluenza virus types 1, 2, and 3 antigens
Protocol tips
• Specimens are incubated with the FITC conjugated reagents for 15 minutes. • Excess reagent is then removed by washing with phosphate-buffered saline (PBS). • The stained areas are mounted and viewed using epifluorescence illumination.
Downstream tips
• Parainfluenza virus types 1, 2, or 3 are present then characteristic apple-green intracellular fluorescence is seen within infected cells, which contrasts with the red background staining of uninfected cells.

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Papers

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for IMAGEN™ Parainfluenza Virus Group Kit using Direct Immunofluorescence Assay below.

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