Wizard® Genomic DNA Purification Kit

Protocol tips

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	For blood DNA isolation, blood must be collected in EDTA, heparin or citrate anticoagulant tubes to prevent clotting.

Protocol tips
For blood DNA isolation, blood must be collected in EDTA, heparin or citrate anticoagulant tubes to prevent clotting.

Publication protocol

2.4. Placental evaluation
2.4.1.
The placenta was collected after delivery and was either paraffin-embedded for immunohistochemistry or flash-frozen in liquid nitrogen for Quantitative Polymerase chain reaction (Q-PCR). DNA was isolated from the placenta tissues, membrane, and cord using a Wizard Genomic DNA Purification kit (Promega, Madison, WI, USA). The DNA samples were than analyzed using the absolute quantification real-time PCR method. Samples were run with FastStart Essential DNA Green Master mix (Roche, Indianapolis, IN, USA) and commercially available primers and controls: β-actin [8] and Coccidioides species [9]. The assays were performed in triplicate. A standard curve was prepared from dilutions of a Coccidioides immitus control DNA (Vircell, Granda, Spain). The dilutions used to make the standard curve were 60,000 copies/rxn, 6000 copies/rxn, 600 copies/rxn, and 60 copies/rxn.

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Manufacturer protocol

Download the product protocol from Promega for Wizard® Genomic DNA Purification Kit below.

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