Wizard® Genomic DNA Purification Kit

DNA isolation / purification Tissue - placenta

Experiment
DNA isolation / purification Tissue - placenta
Product
Wizard® Genomic DNA Purification Kit from Promega
Manufacturer
Promega

Protocol tips

Protocol tips
For blood DNA isolation, blood must be collected in EDTA, heparin or citrate anticoagulant tubes to prevent clotting.

Publication protocol

2.4. Placental evaluation
2.4.1.
The placenta was collected after delivery and was either paraffin-embedded for immunohistochemistry or flash-frozen in liquid nitrogen for Quantitative Polymerase chain reaction (Q-PCR). DNA was isolated from the placenta tissues, membrane, and cord using a Wizard Genomic DNA Purification kit (Promega, Madison, WI, USA). The DNA samples were than analyzed using the absolute quantification real-time PCR method. Samples were run with FastStart Essential DNA Green Master mix (Roche, Indianapolis, IN, USA) and commercially available primers and controls: β-actin [8] and Coccidioides species [9]. The assays were performed in triplicate. A standard curve was prepared from dilutions of a Coccidioides immitus control DNA (Vircell, Granda, Spain). The dilutions used to make the standard curve were 60,000 copies/rxn, 6000 copies/rxn, 600 copies/rxn, and 60 copies/rxn.

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Reviews

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Discussion

Discussion

5 years ago

Author: Denmark

What DNA isolation kit would work for insect samples?

Hello everyone! I am currently using different DNA isolation kits to extract DNA from insects. Even though I am able to successfully extract DNA I would like to maximize the yield. Do you have any tips that might help me with that even if the kits are not specifically designed for insect samples?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA isolation / purification Tissue - placenta using Wizard® Genomic DNA Purification Kit from Promega.

Paper title
Coccidioidomycosis in pregnancy: Case report and literature review of associated placental lesions
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Manufacturer protocol

Download the product protocol from Promega for Wizard® Genomic DNA Purification Kit below.

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Videos

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