GFP-CERTIFIED® Apoptosis/Necrosis detection kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
• Kit contained 7-aminoactinomycin D (7-AAD) and Annexin V for the detection of necroptosis and apoptosis, respectively.	• Applications: Flow Cytometry, Fluorescence microscopy, Fluorescent detection

Upstream tips
• Kit contained 7-aminoactinomycin D (7-AAD) and Annexin V for the detection of necroptosis and apoptosis, respectively.
Protocol tips
• Applications: Flow Cytometry, Fluorescence microscopy, Fluorescent detection

Publication protocol

Necroptosis was also analyzed using a Green Fluorescent Protein-Certified Apoptosis/Necrosis Detection kit (cat. No. ENZ-51002; Enzo Life Sciences), according to the manufacturer’s protocol. This kit contained 7-aminoactinomycin D (7-AAD) and Annexin V for the detection of necroptosis and apoptosis, respectively. In brief, cells were plated into 8-well chamber slides for 24 h of serum starvation. Cells were observed under a confocal microscope (×200) after staining with 7-aminoactinomycin D (7-AAD) to identify necroptotic cells coated in red color and Annexin V-Enzo Gold (enhanced Cyanine-3) to detect apoptotic cells-stained yellow color.

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Manufacturer protocol

Download the product protocol from Enzo Life Sciences for GFP-CERTIFIED® Apoptosis/Necrosis detection kit below.

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