Apoptosis/ Necrosis Assay Kit (blue, green, red) (ab176749)

Necrosis MCF7

Experiment

Necrosis MCF7

Product

Apoptosis/ Necrosis Assay Kit (blue, green, red) (ab176749) from Abcam

Manufacturer

Abcam

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Protocol tips

Upstream tips	Protocol tips	Downstream tips
• Assay type: Cell-based • Platform: Flow cytometer, Fluorescence microscope • Assay time: 1 hr • Sample type: Adherent cells, Suspension cells	• The Kit is designed to simultaneously monitor apoptotic, necrotic, and healthy cells with a flow cytometer or fluorescence microscope. In apoptotic cells, PS is transferred to the outer side of the plasma membrane and can be detected by its sensor, Apopxin Green (green fluorescence). The 7-aminoactinomycin D (red fluorescence), a membrane-impermeable dye, will label the nucleus of damaged cells, including both late apoptotic and necrotic cells. Live cells can be detected as blue fluorescence by CytoCalcein Violet 450 staining.

Upstream tips
• Assay type: Cell-based • Platform: Flow cytometer, Fluorescence microscope • Assay time: 1 hr • Sample type: Adherent cells, Suspension cells
Protocol tips
• The Kit is designed to simultaneously monitor apoptotic, necrotic, and healthy cells with a flow cytometer or fluorescence microscope. In apoptotic cells, PS is transferred to the outer side of the plasma membrane and can be detected by its sensor, Apopxin Green (green fluorescence). The 7-aminoactinomycin D (red fluorescence), a membrane-impermeable dye, will label the nucleus of damaged cells, including both late apoptotic and necrotic cells. Live cells can be detected as blue fluorescence by CytoCalcein Violet 450 staining.

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Papers

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Manufacturer protocol

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