TACS Annexin V-FITC Apoptosis Detection Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
• TACS Annexin V-FITC Apoptosis Detection Kit is supplied with an optimized Binding Buffer and propidium iodide	• Seed 5×10^4/cells per well

Upstream tips
• TACS Annexin V-FITC Apoptosis Detection Kit is supplied with an optimized Binding Buffer and propidium iodide
Protocol tips
• Seed 5×10^4/cells per well

Publication protocol

Two negative controls—cells without any treatment and cells treated with DMSO—were used. Apoptosis/necrosis was monitored using the TACS®Annexin V–FITC Apoptosis Detection Kit according to the protocol provided by the manufacturer. The percentage of apoptotic/necrotic cells was measured by flow cytometry using a Facs Calibur flow cytometer (BD Biosciences, San Jose, CA, USA).

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Manufacturer protocol

Download the product protocol from R&D Systems for TACS Annexin V-FITC Apoptosis Detection Kit below.

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