GFP-CERTIFIED® Apoptosis/Necrosis detection kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
• This kit contained 7-amino-actinomycin D (7-AAD) and Annexin V, which were used to detect necroptosis and apoptosis, respectively.	• Cells were observed under a confocal microscope (×200) after staining with 7-aminoactinomycin D (7-AAD) to identify necroptotic cells coated in red color.

Upstream tips
• This kit contained 7-amino-actinomycin D (7-AAD) and Annexin V, which were used to detect necroptosis and apoptosis, respectively.
Protocol tips
• Cells were observed under a confocal microscope (×200) after staining with 7-aminoactinomycin D (7-AAD) to identify necroptotic cells coated in red color.

Publication protocol

Necroptosis was also analyzed using a Green Fluorescent Protein-Certified Apoptosis/Necrosis Detection kit (cat. No. ENZ-51002; Enzo Life Sciences), according to the manufacturer’s protocol. This kit contained 7-aminoactinomycin D (7-AAD) and Annexin V for the detection of necroptosis and apoptosis, respectively. In brief, cells were plated into 8-well chamber slides for 24 h of serum starvation. Cells were observed under confocal microscope (×200) after staining with 7-aminoactinomycin D (7-AAD) to identify necroptotic cells coated in red color and Annexin V-EnzoGold (enhanced Cyanine-3) to detect apop-totic cells stained yellow color.

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Manufacturer protocol

Download the product protocol from Enzo Life Sciences for GFP-CERTIFIED® Apoptosis/Necrosis detection kit below.

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