Apoptosis/ Necrosis Assay Kit (blue, green, red) (ab176749)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
• Assay type: Cell-based • Platform: Flow cytometer, Fluorescence microscope • Assay time: 1 hr • Sample type: Adherent cells, Suspension cells	• 1×10^4/well in flat-bottom 96-well culture plates

Upstream tips
• Assay type: Cell-based • Platform: Flow cytometer, Fluorescence microscope • Assay time: 1 hr • Sample type: Adherent cells, Suspension cells
Protocol tips
• 1×10^4/well in flat-bottom 96-well culture plates

Publication protocol

Cells were seeded at 1×10^4/well in flat-bottom 96-well culture plates and allowed to grow for 24 h, followed by incubation with HER2 Affibody–IR700Dye conjugate (0.5μM) for 2 h at 37◦C. After washing the cells twice with PBS, near-infrared (NIR) light (60 J/cm2) was irradiated from the bottom of wells. Then, apoptosis or necrosis of the cells was determined using the Apoptosis/Necrosis Assay Kit (ab176749, Abcam, Cambridge, UK) as the manufacturing protocol described.

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Manufacturer protocol

Download the product protocol from Abcam for Apoptosis/ Necrosis Assay Kit (blue, green, red) (ab176749) below.

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