Alexa Fluor® 488 Annexin V/Dead Cell Apoptosis Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
• Kit contains recombinant annexin V conjugated to one of our best and brightest fluorophores, the Alexa Fluor® 488 dye, to provide maximum sensitivity. • Kit includes a ready-to-use solution of the red-fluorescent propidium iodide (PI) nucleic acid binding dye.	• The Alexa Fluor® 488 annexin V and propidium iodide are light sensitive and may be handled in normal room light, but avoid prolonged exposure to light. • Alexa Fluor® 488 annexin V: 488/499 in nm; • Propidium iodide: 535/617 in nm, bound to DNA.

Upstream tips
• Kit contains recombinant annexin V conjugated to one of our best and brightest fluorophores, the Alexa Fluor® 488 dye, to provide maximum sensitivity. • Kit includes a ready-to-use solution of the red-fluorescent propidium iodide (PI) nucleic acid binding dye.
Protocol tips
• The Alexa Fluor® 488 annexin V and propidium iodide are light sensitive and may be handled in normal room light, but avoid prolonged exposure to light. • Alexa Fluor® 488 annexin V: 488/499 in nm; • Propidium iodide: 535/617 in nm, bound to DNA.

Publication protocol

Following the exposure, 1 million cells were harvested, washed and labelled with Alexa Fluor® 488-Annexin V and 7-AAD as directed by the manufacturer. The samples were analyzed by a guava easyCyte® 8HT (Millipore) with 10,000 events collected for each sample. In parallel, 10,000 K562 cells per well were dosed with a threefold dilution series of bortezomib in the presence of the bioluminescent annexin and necrosis detection reagent.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Alexa Fluor® 488 Annexin V/Dead Cell Apoptosis Kit below.

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