QIAamp DNA Blood Mini Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
		- Include RNAse treatment for 15-20 min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C - Use prewarmed TE buffer to elute the DNA

Downstream tips
- Include RNAse treatment for 15-20 min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C - Use prewarmed TE buffer to elute the DNA

Publication protocol

Isolation of SRV-4 from SRV-4 proviral DNA-positive Japanese macaques.
Concanavalin A-stimulated PBMCs were cocultured with HEK293T cells. Two weeks after cocultivation, supernatants of the cocultured cells were filtered and then inoculated into uninfected HEK293T cells. In parallel, the plasma samples were inoculated into HEK293T cells and cultured. Two weeks after inoculation, genomic DNAs were isolated from the inoculated cells using a QIAamp DNA blood minikit (Qiagen, Valencia, CA) and then subjected to PCR analysis as described below.

Isolation of SRV-4 from a Japanese macaque exhibiting thrombocytopenia.
PBMCs or BM cells were cocultured with TELCeB6 cells. In parallel, the plasma sample or stool suspension was inoculated into TELCeB6 cells. Two weeks after inoculation or cocultivation, each culture supernatant containing 8 μg/ml of Polybrene (hexadimethrine bromide) (Sigma-Aldrich) was filtered through a 0.45-μm filter unit (Pall) and then inoculated into uninfected TE671 cells. Two days after inoculation, cells were stained with X-Gal (5-bromo-4-chloro-3-indolyl-β-d-galactopyranoside). In addition, the genomic DNAs were isolated from all inoculated cells using a QIAamp DNA blood minikit (Qiagen) and then subjected to PCR analysis as described below.



Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA isolation / purification Cells - Immortalized cell lines HEK 293T using QIAamp DNA Blood Mini Kit from Qiagen.

Manufacturer protocol

Download the product protocol from Qiagen for QIAamp DNA Blood Mini Kit below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing DNA isolation / purification Cells - Immortalized cell lines HEK 293T using QIAamp DNA Blood Mini Kit from Qiagen. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.