Cell Proliferation Kit (XTT based)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Easy-to-use: There is no requirement for additional reagents and/or the cell washing procedures Speed: Multiwell plates and an ELISA reader can be used for reading Sensitivity: Can be assayed even in low cell concentrations Accuracy: Dye absorbance is proportional to the number of cells in each well Safety: There is no need for radioactive isotopes	Kit was developed for cell proliferation assay in reaction to different growth factors, cytokines and nutrient components. It is suitable for assaying cytotoxicity of materials such as TNF or other growth inhibitors and for lymphocyte activation. Kit is an excellent solution for the quantitating of cell proliferation and viability, without using radioactive isotopes also for non-radioactive substitute for cytotoxic tests based on the release of 51Cr from cells, maintaining high sensitivity.	XTT results were blotted and the dose needed to kill 50% of the cells was determined using Prism software.

Upstream tips
Easy-to-use: There is no requirement for additional reagents and/or the cell washing procedures Speed: Multiwell plates and an ELISA reader can be used for reading Sensitivity: Can be assayed even in low cell concentrations Accuracy: Dye absorbance is proportional to the number of cells in each well Safety: There is no need for radioactive isotopes
Protocol tips
Kit was developed for cell proliferation assay in reaction to different growth factors, cytokines and nutrient components. It is suitable for assaying cytotoxicity of materials such as TNF or other growth inhibitors and for lymphocyte activation. Kit is an excellent solution for the quantitating of cell proliferation and viability, without using radioactive isotopes also for non-radioactive substitute for cytotoxic tests based on the release of 51Cr from cells, maintaining high sensitivity.
Downstream tips
XTT results were blotted and the dose needed to kill 50% of the cells was determined using Prism software.

Publication protocol

To test the effect on cell proliferation, 1500 cells of different cancer cell lines were seeded
in triplicates in 96-well plates. 24 h later, cells were treated with different concentrations of
NO–curcumin or curcumin. In the following days, the difference in cell proliferation rates
was tested using a commercially available XTT kit (Biological industries, Israel) according
to the manufacturer’s instructions. In order to determine the IC50, XTT results were blotted
and the dose needed to kill 50% of the cells was determined using Prism software.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Cell cytotoxicity / Proliferation assay cell type - HCT116 using Cell Proliferation Kit (XTT based) from SARTORIUS.

Manufacturer protocol

Download the product protocol from SARTORIUS for Cell Proliferation Kit (XTT based) below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Cell cytotoxicity / Proliferation assay cell type - HCT116 using Cell Proliferation Kit (XTT based) from SARTORIUS. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.