RealTime-Glo™ Annexin V Apoptosis and Necrosis Assay
Necrosis LNCaP
Publication protocol
The cells were seeded in 96-well plates at a density of 2000 cells/well, adhered overnight, then 50 nM DHT was added and cultured at 37 °C, 5% CO2for 48 h. Then, we followed the steps of RealTime-Glo™Annexin V Apoptosis and necrosis Assay Kit (Promega) to detect apoptosis and necrosis. EtOH was used as the negative control and H2O2was used as the positive control of the 6 h treatment.Full paper Login or join for free to view the full paper.
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