Apoptosis/ Necrosis Assay Kit (blue, green, red) (ab176749)

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Briefly, fluorescent analysis shows apoptotic (green, Apopxin Green Indicator), and necrotic cells (red, indicated by 7AADstaining). Positive controls have been obtained by cell treatment with 1μM staurosporine for 3h. The fluorescence images of the cells were taken with a fluorescent microscope through the FITC and TRITC channels. Individual images taken from each channel from the same cell population are merged. Live cell cytoplasm labeling dye, Cyto Calcein Violet450 (Ex/Em=405/450nm), was used for labeling living cell cytoplasm. Data are expressed as Ncells/field (medianvaluesofthe10fieldsanalysed: green cells are the apoptotic cells; red cells are the necrotic cells). We considered the number of apoptotic cells/fields (Ncell/fields) togetherwith%ofapo-ptotic/total death cells, to compare apoptosis/necrosis in control and Apixaban incubated cells (5μg/ml,96-h).

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