EZ DNA Methylation kit

DNA methylation profiling Whole genome profiling - mouse hippocampal tissue

Experiment
DNA methylation profiling Whole genome profiling - mouse hippocampal tissue
Product
EZ DNA Methylation kit from Zymo Research
Manufacturer
Zymo Research

Protocol tips

Upstream tips
For best results, the CT Conversion Reagent should be prepared freshly and used immediately following preparation.
Protocol tips
Always include fully methylated (in-vitro methylated DNA) and non-methylated controls (Leucocyte DNA, DNMT1/3 double KO DNA) during MSP

Publication protocol

2.6. DNA methylation analyses
Genomic DNA was extracted from cells using GenElute™ (Sigma–Aldrich, St. Louis, Missouri, USA) and from rat brain tissues using the AllPrep DNA/RNA/Protein Mini Kit (Qiagen, Hilden, Germany) following manufacturer's instructions. Bisulfite conversion was performed using EZ Methylation Gold Kit (Zymo Research, Irvine, California, USA) and Nr3c1 promoter, Fkbp5 intron 5 and intron 1was amplified by nested PCR (for primer sequences (Supplemental material, Table S1). Pyrosequencing was performed with 25 μl of the resulting PCR product in a Pyromark Q96 ID using Pyromark Q96 Gold reagents (Qiagen, Hilden, Germany) according to the manufacturer's instructions.

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Papers

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Paper title
Developmental exposure to bisphenol A alters expression and DNA methylation of Fkbp5, an important regulator of the stress response.
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Manufacturer protocol

Download the product protocol from Zymo Research for EZ DNA Methylation kit below.

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