MethylFlash™ Methylated DNA Quantification Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Always include fully methylated (in-vitro methylated DNA) and non-methylated controls (Leucocyte DNA, DNMT1/3 double KO DNA) during MSP

Upstream tips
Always include fully methylated (in-vitro methylated DNA) and non-methylated controls (Leucocyte DNA, DNMT1/3 double KO DNA) during MSP

Publication protocol

Global DNA methylation status detection and calculation
The MethylFlash™ Methylated DNA Quantification Kit (Fluorometric) was used to detect the total amount of 5mC in the thalamus and hippocampus according to the manufacturer’s instructions (Epigentek, Farmingdale, NY). Relative fluorescence units (RFU) at 530EX/590EMnm were measured using a SpectraMax M5 (Molecular Devices, CA, USA) to calculate the global methylation status.

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA methylation profiling Whole genome profiling - mouse hippocampal tissue using MethylFlash™ Methylated DNA Quantification Kit from Epigentek.

Manufacturer protocol

Download the product protocol from Epigentek for MethylFlash™ Methylated DNA Quantification Kit below.

Download manufacturer protocol

Videos

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