EZ DNA Methylation kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
For best results, the CT Conversion Reagent should be prepared freshly and used immediately following preparation.

Upstream tips
For best results, the CT Conversion Reagent should be prepared freshly and used immediately following preparation.

Publication protocol

DNA methylation analysis
Genomic DNA was extracted from C2C12 cells using the Genomic DNA Clean & Concentrator kit (Zymo Research). Bisulfite treatment and recovery of samples were carried out with the EZ DNA Methylation-Gold Kit (Zymo Research) according to the manufacturer’s instructions. Bisulfite sequencing primers were designed with the online MethPrimer software (Supplementary Table S1). PCR was performed with the Hot-Start Ex Taq DNA Polymerase (TAKARA), and the products were gel selected, purified using the TIANgel Midi Purification Kit (Tiangen Biotech) and cloned through TA cloning using the pEASY-T1 Simple Cloning Kit (TransGen Biotech). At least 10 clones were sequenced and the results were analyzed with BiQ Analyzer software.

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Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA methylation profiling Whole genome profiling - C2C12 mouse myoblast cells using EZ DNA Methylation kit from Zymo Research.

Manufacturer protocol

Download the product protocol from Zymo Research for EZ DNA Methylation kit below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing DNA methylation profiling Whole genome profiling - C2C12 mouse myoblast cells using EZ DNA Methylation kit from Zymo Research. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.