Imprint® Methylated DNA Quantification Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Ensure DNA is mixed thoroughly prior to application. The wells of the assay strip bind up to 200 ng of total DNA.

Protocol tips
Ensure DNA is mixed thoroughly prior to application. The wells of the assay strip bind up to 200 ng of total DNA.

Publication protocol

Global DNA Methylation Assay
Genomic DNA was isolated from undenervated and denervated TA muscle of mice or C2C12 myoblasts using DNeasy® blood and tissue kit (Qiagen). Global DNA methylation was assayed using an ELISA-based commercial kit (MDQ1; Imprint methylated DNA quantification kit; Sigma-Aldrich). Two microliters genomic DNA at a concentration of 100 ng/μl was diluted with 28 μl of lysis and binding buffers. After incubation with the detection antibody, absorbance was read at 450 nm. Positive (methylated) and negative (unmethylated) control DNA was supplied with the kit. Global DNA methylation level was calculated as a percentage relative to methylated control DNA. All of the samples were analyzed in duplicate.

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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for Imprint® Methylated DNA Quantification Kit below.

Download manufacturer protocol

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