EZ DNA Methylation kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
For best results, the CT Conversion Reagent should be prepared freshly and used immediately following preparation.

Upstream tips
For best results, the CT Conversion Reagent should be prepared freshly and used immediately following preparation.

Publication protocol

Bisulfite sequencing and combined bisulfite restriction analysis (COBRA)
500 ng of genomic DNA from each mouse liver tissue was treated with bisulfite using EZ DNA Methylation-Gold Kit (Zymo Research, Orange, CA, USA) followed by PCR. The primers used were listed in . For bisulfite sequencing, the PCR products of three control or TCE treated samples were pooled and cloned into TA-cloning vector (Tiangen), and individual clones were sequenced using an automatic sequencer (ABI 3730XL). For COBRA, the PCR products were digested with the corresponding restriction enzymes and analyzed on 2% agarose gel as described earlier .

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Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA methylation profiling Whole genome profiling - mouse liver tissue using EZ DNA Methylation kit from Zymo Research.

Manufacturer protocol

Download the product protocol from Zymo Research for EZ DNA Methylation kit below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing DNA methylation profiling Whole genome profiling - mouse liver tissue using EZ DNA Methylation kit from Zymo Research. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.