JetPrime

Protocol tips

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jetPRIME® is stable in presence of serum and antibiotics therefore you may use serum and antibiotic containing medium during the entire experiment.

Upstream tips
jetPRIME® is stable in presence of serum and antibiotics therefore you may use serum and antibiotic containing medium during the entire experiment.

Publication protocol

Cells were seeded (150,000 cells/well) on six-well culture dishes to 30–50% confluence and transfected with 5–100 nmol/l siRNA-hVDAC1 using the JetPRIME reagent in the case of HeLa and A549, PC-3, HepG2, U87, HaCat, and Min-6 and Panc-1cells, while HCT116 and HEK293, cells were transfected using Dharmafect, all according to the manufacturers' instructions.

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Papers

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Manufacturer protocol

Download the product protocol from Polyplus transfections for JetPrime below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing DNA transfection Mammalian cells - Immortalized cell lines PANC-1 using JetPrime from Polyplus transfections. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.