Lipofectamine® 2000 Transfection Reagent

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Do not add antibiotics to the Transfection Medium

Protocol tips
Do not add antibiotics to the Transfection Medium

Publication protocol

MCF-7 cell transfection
According to the instructions for the transfection reagent, MCF-7 cells were plated 24 h before transfection and were continuously incubated until 60% ∼ 70% confluency. Then, cells were transfected by groups. Before transfection, cells were serum starved in FBS-free DMEM solution for 1 h. Transfection compounds were prepared for each well, in accordance with the instructions for the Lipofectamine 2000 kit (Invitrogen Inc., Carlsbad, CA, USA). The control group was incubated with serum-free, antibiotic-free medium, while the HAS-2-siRNA group was incubated with serum-free, antibiotic-free medium containing HAS-2 siRNA (the final concentration was 20 umol/L), which was enclosed in lipidosomes (Invitrogen Inc., Carlsbad, CA, USA); the scrambled-siRNA group was incubated with serum-free, antibiotic-free medium containing scrambled-siRNA (the final concentration was 20 umol/L) that was enclosed in lipidosomes. The transfected cell cultures were blocked for 4 h in serum-free medium, after which 10% FBS was added, and the cells were cultured in a 5% CO2 incubator at 37°C.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Lipofectamine® 2000 Transfection Reagent below.

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