FuGENE® HD Transfection Reagent

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Always bring FuGENE HD to room temperature and mix well prior to use.

Upstream tips
Always bring FuGENE HD to room temperature and mix well prior to use.

Publication protocol

Cell transfection and proliferation assay
Approximately 1.0 × 10 cells were plated and the plasmids were transfected using the FuGENE HD Transfection Reagent (Promega) according to the manufacturer's protocol. Cell proliferation was measured at 490 nm absorbance after incubation for 1 h in Cell Titer 96 Aqueous One Solution (Pro-mega), containing 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt (MTS) solution.



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Papers

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Manufacturer protocol

Download the product protocol from Promega for FuGENE® HD Transfection Reagent below.

Download manufacturer protocol

Videos

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