FuGENE® HD Transfection Reagent

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Always bring FuGENE HD to room temperature and mix well prior to use.

Upstream tips
Always bring FuGENE HD to room temperature and mix well prior to use.

Publication protocol

ISONA150 was tested in two kinds of liver cancer cell lines—Huh7 and Hep3b with or without FuGENE (Fig. 2). FuGENE is commonly used in labs to facilitate a cell’s uptake of DNAs. In Huh7, cells with more DNA added have higher radioactivity and there was no significant difference with a different concentration of FuGENE. The data here suggested that uptake of ISONA is independent of FuGENE but related to the amount of DNA. In Hep3b cells, the uptake is much lower compared with Huh7.

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Manufacturer protocol

Download the product protocol from Promega for FuGENE® HD Transfection Reagent below.

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