jetPEI® DNA transfection, HTS application

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Do not add antibiotics to the Transfection Medium

Protocol tips
Do not add antibiotics to the Transfection Medium

Publication protocol

Cells were plated at a density of 1.105 cells per well in 24-well plates and grown for 24 h to 80% confluence. Transfections using TransMessenger™ (Qiagen, Hilden, Germany) or TransIT®mRNA (Mirus Bio, Madison WI, USA) were performed following the manufacturer’s instructions. Transfections using jetPEI™ or fluorescein-labeled jetPEI™-FluoF (Polyplus Transfection, Illkirch, France) were performed at an N/P ratio of 5 in Opti-MEM (Invitrogen) following the Manufacturer’s protocol. When mRNA and pDNA were co-transfected, complexes were formed separately with the transfection reagent to avoid mRNA degradation due to RNases potentially present in the pDNA preparation. Cells were then incubated at 37°C for 30 min to 48 h, depending on the experiment.

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Manufacturer protocol

Download the product protocol from Polyplus transfections for jetPEI® DNA transfection, HTS application below.

Download manufacturer protocol

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