jetPEI® DNA transfection, HTS application

DNA transfection Mammalian cells - Immortalized cell lines HeLa

Experiment
DNA transfection Mammalian cells - Immortalized cell lines HeLa
Product
jetPEI® DNA transfection, HTS application from Polyplus transfections
Manufacturer
Polyplus transfections

Protocol tips

Protocol tips
Do not add antibiotics to the Transfection Medium

Publication protocol

Cells were plated at a density of 1.105 cells per well in 24-well plates and grown for 24 h to 80% confluence. Transfections using TransMessenger™ (Qiagen, Hilden, Germany) or TransIT®mRNA (Mirus Bio, Madison WI, USA) were performed following the manufacturer’s instructions. Transfections using jetPEI™ or fluorescein-labeled jetPEI™-FluoF (Polyplus Transfection, Illkirch, France) were performed at an N/P ratio of 5 in Opti-MEM (Invitrogen) following the Manufacturer’s protocol. When mRNA and pDNA were co-transfected, complexes were formed separately with the transfection reagent to avoid mRNA degradation due to RNases potentially present in the pDNA preparation. Cells were then incubated at 37°C for 30 min to 48 h, depending on the experiment.

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA transfection Mammalian cells - Immortalized cell lines HeLa using jetPEI® DNA transfection, HTS application from Polyplus transfections.

Paper title
Exogenous mRNA delivery and bioavailability in gene transfer mediated by piggyBac transposition
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Manufacturer protocol

Download the product protocol from Polyplus transfections for jetPEI® DNA transfection, HTS application below.

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