PolyFect Transfection Reagent

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Up to 50–100 transfections in 6-well plates / 1 ml reagent

Upstream tips
Up to 50–100 transfections in 6-well plates / 1 ml reagent

Publication protocol

Plasmid constructs and reagents
The × 1.3 Cp-luciferase HBV was generously provided by Y. Shaul (Weizmann Institute of Science, Rehovot, Israel). The 1.2 mer HBV including N-terminal × 3 flagged HBx were kindly provided by W. S. Ryu (Yonsei University, Seoul, South Korea). To construct HBV-Xp-luc and HBV-preS1p-luc, the promoter fragments in HBV genome construct were amplified by polymerase chain reaction (PCR) using cloning primers containing restriction enzyme site, HindIII, and KpnI. After digestion, the fragments were cloned into pGL4 vectors. The sequences were confirmed by automated DNA sequencing. Sorafenib was purchased from Selleckchem (Houston, TX, USA). Chenodeoxycholic acid (CDCA) and Z-guggulsterone (Z-GGS) were purchased from Sigma (St. Louis, MO, USA). SP600125 was purchased from Calbiochem (Billerica, MA, USA). The transfection reagents PolyFect and jetPEI were purchased from Qiagen (Hilden, Germany).

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Manufacturer protocol

Download the product protocol from Qiagen for PolyFect Transfection Reagent below.

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