Attractene Transfection Reagent

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	The ovarian carcinoma cells were treated with RhoC-expressing plasmid by Attractene Transfection Reagent (QIAGEN) with pEGFP-N1 as a mock or RhoC siRNA (Sigma, USA) by HiPerFect Transfection Reagent (QIAGEN).

Protocol tips
The ovarian carcinoma cells were treated with RhoC-expressing plasmid by Attractene Transfection Reagent (QIAGEN) with pEGFP-N1 as a mock or RhoC siRNA (Sigma, USA) by HiPerFect Transfection Reagent (QIAGEN).

Publication protocol

The ovarian carcinoma cells were treated with RhoC-expressing plasmid by Attractene Transfection Reagent (QIAGEN) with pEGFP-N1 as a mock or RhoC siRNA (Sigma, USA) by HiPerFect Transfection Reagent (QIAGEN). The target sequences of RhoC siRNA were 5′-GUGCCUUUGGCUACCUUGAdTdT-3′ (sense) and 5′-UCAAGGUAGCCAAAGGCA CdTdT-3′ (anti-sense). The negative siRNA control sequences were 5′-UUCUCCGAACGU GUCACGUT T-3′ (sense) and 5′-ACGUGACACGUUCGGAGAATT-3′ (anti-sense). Cells were treated by recombinant human TGF-β1 and VEGF165 (Perotech), VEGF receptor inhibitor BIBF1120 and TGF-β1 receptor inhibitor SB431542 (Selleckchem). All cells were harvested by centrifugation, rinsed with phosphate buffered saline (PBS), and subjected to RNA and protein extraction.

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