FuGENE® HD Transfection Reagent

Protocol tips

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	Plasmid transfections of 3×105 OVCAR3 cells seeded in 6-well plates were performed in antibiotic-free growth medium using FuGENE HD (Roche Diagnostic Corporation; Indianapolis, IN). Controls were treated with 100 ul/ml OPTI-MEM only and vehicle controls with 3ul/ml FuGENE HD

Protocol tips
Plasmid transfections of 3×105 OVCAR3 cells seeded in 6-well plates were performed in antibiotic-free growth medium using FuGENE HD (Roche Diagnostic Corporation; Indianapolis, IN). Controls were treated with 100 ul/ml OPTI-MEM only and vehicle controls with 3ul/ml FuGENE HD

Publication protocol

The plasmid pDsRed2-Bid for expression of DsRed fused to the C-terminus of Bid to allow translocation analyses of full-length Bid and tBid was purchased commercially (Clontech Laboratories, Inc., Mountain View, CA). Plasmid transfections of 3×105 OVCAR3 cells seeded in 6-well plates were performed in antibiotic-free growth medium using FuGENE HD (Roche Diagnostic Corporation; Indianapolis, IN). Controls were treated with 100 ul/ml OPTI-MEM only and vehicle controls with 3ul/ml FuGENE HD.

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Manufacturer protocol

Download the product protocol from Promega for FuGENE® HD Transfection Reagent below.

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