TransIT®-LT1 Transfection Reagent

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Always add your plasmid to the buffer before adding TransIT

Upstream tips
Always add your plasmid to the buffer before adding TransIT

Publication protocol

EB3 microtubule tracking
To visualize live-cell microtubule dynamics, cells were transfected with mApple-EB3, a gift from Michael Davidson (Addgene plasmid #54892), using the TransIT-LT1 transfection reagent (Mirus Bio) per manufacturer's instructions. Cells were imaged with a Nikon Eclipse Ti inverted epifluorescent microscope, maintained at 37°C and 5% carbon dioxide throughout the experiment using an In Vivo Scientific environmental cell chamber and Bioscience Tools CO2 controller. A Photometrics QuantEM CCD camera (Princeton Instruments) was used to minimize exposure time while imaging with a Nikon CFI Apochromat TIRF 100X oil-immersion lens. Videos were captured at 1 Hz for 2 minutes and quantified using the open source u-track software as described27,67.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA transfection Mammalian cells - Immortalized cell lines SKOV-3 using TransIT®-LT1 Transfection Reagent from Mirus.

Manufacturer protocol

Download the product protocol from Mirus for TransIT®-LT1 Transfection Reagent below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing DNA transfection Mammalian cells - Immortalized cell lines SKOV-3 using TransIT®-LT1 Transfection Reagent from Mirus. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.