FuGENE® HD Transfection Reagent

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Always bring FuGENE HD to room temperature and mix well prior to use.

Upstream tips
Always bring FuGENE HD to room temperature and mix well prior to use.

Publication protocol

Cell lines and culture conditions
We purchased SKOV3 cells from ATCC (Manassas, VA). The cells were maintained in Dulbecco's Modified Eagle Medium (DMEM) containing 10% fetal calf serum, 2 mM L-Glutamine, and an antibiotic combination of 0.1 mg/ml streptomycin and 100 U/ml penicillin (all from Invitrogen, Carlsbad, CA). The cells were incubated at 37°C in a humidified air atmosphere containing 5% CO2. For transfection, 1 × 106 SKOV3 cells were plated onto a 6-well plate in DMEM without antibiotics approximately 24 hours prior to transfection. A mix of 2 μg left and 2 μg right TALEN plasmids were co-transfected into SKOV3 cells using the FuGENE HD Transfection Reagent (Promega, Madison, WI) following the manufacturer's protocol.

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Reviews

Discussion

Papers

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Manufacturer protocol

Download the product protocol from Promega for FuGENE® HD Transfection Reagent below.

Download manufacturer protocol

Videos

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