Lipofectamine® 2000 Transfection Reagent

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Do not add antibiotics to the Transfection Medium

Protocol tips
Do not add antibiotics to the Transfection Medium

Publication protocol

Plasmid and transient transfection
The antisense plasmid pEGFP-C1-mTOR was donated by Dr Yu Guo (Liver Transplant Center, The Third Affiliated Hospital of Sun Yat-Sen University). Transient transfection was conducted using Lipofectamine® 2000 reagent (Invitrogen; Thermo Fisher Scientific, Inc.) according to the manufacturer's protocol. SMMC-7721 cells were seeded in 24-well plates (5×104 cells/well) one day prior to transfection until they reached 80% confluence. A total of 1 µg plasmid DNA and 3 µl transfection reagent were used to transfect the cells in each well in the absence of serum. After 4 h, the medium was replaced with DMEM supplemented with 10% FBS. The expression of green fluorescent protein was observed using the TE2000-U inverted fluorescence microscope.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA transfection Mammalian cells - Immortalized cell lines SMMC-7721 using Lipofectamine® 2000 Transfection Reagent from Thermo Fisher Scientific.

Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Lipofectamine® 2000 Transfection Reagent below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing DNA transfection Mammalian cells - Immortalized cell lines SMMC-7721 using Lipofectamine® 2000 Transfection Reagent from Thermo Fisher Scientific. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.