jetPEI® DNA transfection, HTS application

Protocol tips

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	Do not add antibiotics to the Transfection Medium

Protocol tips
Do not add antibiotics to the Transfection Medium

Publication protocol

ARE luciferase reporter vector, siRNA, and vector of Nrf2 and Keap1 were designed and purchased from GeneChem (NM 001145413). siNrf2, 5′-GGCATTTCACTAAACACAA-3′; siKeap1, 5′-CTTAATTCAGCTGAGTGTT-3′. The vectors were transfected into HCC cells with jetPEI DNA transfection reagent (Polyplus). Nrf2 cDNA was cloned from the whole cDNA synthesized by total RNA and constructed into plenti-EF3.

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Manufacturer protocol

Download the product protocol from Polyplus transfections for jetPEI® DNA transfection, HTS application below.

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