Tempus™ Spin RNA Isolation Kit

RNA isolation / purification Bacteria - Gram negative Neisseria gonorrhoeae

Experiment

RNA isolation / purification Bacteria - Gram negative Neisseria gonorrhoeae

Product

Tempus™ Spin RNA Isolation Kit from Thermo Fisher Scientific

Manufacturer

Thermo Fisher Scientific

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Protocol tips

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	RNA was isolated in two mixed batches containing PID patients and controls using Tempus Spin RNA Isolation kit at the Clinical Trial Resource Center at the Children's Hospital of Philadelphia using standard manufacturer's instructions. RNA quality was confirmed by Agilent Bioanalyzer assay.

Protocol tips
RNA was isolated in two mixed batches containing PID patients and controls using Tempus Spin RNA Isolation kit at the Clinical Trial Resource Center at the Children's Hospital of Philadelphia using standard manufacturer's instructions. RNA quality was confirmed by Agilent Bioanalyzer assay.

Publication protocol

RNA was isolated in two mixed batches containing PID patients and controls using Tempus Spin RNA Isolation kit at the Clinical Trial Resource Center at the Children's Hospital of Philadelphia using standard manufacturer's instructions. RNA quality was confirmed by Agilent Bioanalyzer assay.

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Discussion

5 years ago

Author: Paul G. Macon United States

Some help with RNA isolation using Trizol

Hello! I used Trizol to extract total RNA from in-vitro cultured bacteria (1 X 10^8 cells). After phase separation, I mixed ~0.4 ml of the upper phase which contains RNA with 0.5 mL cold isopropanol. However, the amount of RNA when measured in Nanodrop was very low. In addition, the ratio between 260 and 230 was around 0.1 to 0.5. Is there a chance that my sample was contaminated by the Trizol reagent? When I collected the aqueous phase I made sure to not touch the lower phase. What should I do?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Bacteria - Gram negative Neisseria gonorrhoeae using Tempus™ Spin RNA Isolation Kit from Thermo Fisher Scientific.

Paper title

RNA Biosignatures in Adolescent Patients in a Pediatric Emergency Department with Pelvic Inflammatory Disease

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Tempus™ Spin RNA Isolation Kit below.

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