Total RNA Purification Plus Kit

RNA isolation / purification Bacteria - Gram negative Vibro cholerae

Experiment

RNA isolation / purification Bacteria - Gram negative Vibro cholerae

Product

Total RNA Purification Plus Kit from Norgen Biotek

Manufacturer

Norgen Biotek

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Tips Publication protocol Reviews Discussion Papers Manufacturer protocol Videos

Protocol tips

Upstream tips	Protocol tips	Downstream tips
For lysis/cell disruption check the protocol beforehand to see which reagents/lab materials are needed		Minimal DNA is left over using this kit, however if DNA is undesired an additional RNase free DNase treatment is warranted.

Upstream tips
For lysis/cell disruption check the protocol beforehand to see which reagents/lab materials are needed
Downstream tips
Minimal DNA is left over using this kit, however if DNA is undesired an additional RNase free DNase treatment is warranted.

Publication protocol

Less yield but cleaner RNA was obtained using the Total RNA Purification Plus Kit (Norgen Biotek corp) according to the manufacturer’s manual followed by treatment with DNase I.

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Reviews

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Discussion

5 years ago

Author: Paul G. Macon United States

Some help with RNA isolation using Trizol

Hello! I used Trizol to extract total RNA from in-vitro cultured bacteria (1 X 10^8 cells). After phase separation, I mixed ~0.4 ml of the upper phase which contains RNA with 0.5 mL cold isopropanol. However, the amount of RNA when measured in Nanodrop was very low. In addition, the ratio between 260 and 230 was around 0.1 to 0.5. Is there a chance that my sample was contaminated by the Trizol reagent? When I collected the aqueous phase I made sure to not touch the lower phase. What should I do?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Bacteria - Gram negative Vibro cholerae using Total RNA Purification Plus Kit from Norgen Biotek.

Paper title

Membrane vesicle-mediated release of bacterial RNA

Full paper

Manufacturer protocol

Download the product protocol from Norgen Biotek for Total RNA Purification Plus Kit below.

Download manufacturer protocol

Videos

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