RiboPure™ RNA Purification Kit, bacteria

RNA isolation / purification Bacteria - Gram negative Salmonella enterica

Experiment

RNA isolation / purification Bacteria - Gram negative Salmonella enterica

Product

RiboPure™ RNA Purification Kit, bacteria from Thermo Fisher Scientific

Manufacturer

Thermo Fisher Scientific

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Tips Publication protocol Reviews Discussion Papers Manufacturer protocol Videos

Protocol tips

Upstream tips	Protocol tips	Downstream tips
This kit involves a bead-beating step, make sure that you have the appropriate equipment		When checking the yield by means of a specrtophotometer, use the supplied TE buffer to zero the machine

Upstream tips
This kit involves a bead-beating step, make sure that you have the appropriate equipment
Downstream tips
When checking the yield by means of a specrtophotometer, use the supplied TE buffer to zero the machine

Publication protocol

The cell pellets were processed for total RNA extraction and DNase treatment using a RiboPure Bacterial Kit (Ambion, USA) according to the protocol supplied by the manufacturer. Total RNA was extracted from three replicates of each strain. Subsequently, equal concentrations of the total-RNA samples obtained from three replicates of each strain were mixed and subjected to a second treatment with DNase to remove any residual genomic DNA, according to the protocols supplied with the RiboPure Bacterial Kit.

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Reviews

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Discussion

5 years ago

Author: Paul G. Macon United States

Some help with RNA isolation using Trizol

Hello! I used Trizol to extract total RNA from in-vitro cultured bacteria (1 X 10^8 cells). After phase separation, I mixed ~0.4 ml of the upper phase which contains RNA with 0.5 mL cold isopropanol. However, the amount of RNA when measured in Nanodrop was very low. In addition, the ratio between 260 and 230 was around 0.1 to 0.5. Is there a chance that my sample was contaminated by the Trizol reagent? When I collected the aqueous phase I made sure to not touch the lower phase. What should I do?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Bacteria - Gram negative Salmonella enterica using RiboPure™ RNA Purification Kit, bacteria from Thermo Fisher Scientific.

Paper title

RNA Sequencing Reveals Differences between the Global Transcriptomes of Serovar Enteritidis Strains with High and Low Pathogenicities

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for RiboPure™ RNA Purification Kit, bacteria below.

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Videos

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