RiboPure™ RNA Purification Kit, bacteria

RNA isolation / purification Bacteria - Gram negative Salmonella enterica

Experiment
RNA isolation / purification Bacteria - Gram negative Salmonella enterica
Product
RiboPure™ RNA Purification Kit, bacteria from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Upstream tips
This kit involves a bead-beating step, make sure that you have the appropriate equipment
Downstream tips
When checking the yield by means of a specrtophotometer, use the supplied TE buffer to zero the machine

Publication protocol

The cell pellets were processed for total RNA extraction and DNase treatment using a RiboPure Bacterial Kit (Ambion, USA) according to the protocol supplied by the manufacturer. Total RNA was extracted from three replicates of each strain. Subsequently, equal concentrations of the total-RNA samples obtained from three replicates of each strain were mixed and subjected to a second treatment with DNase to remove any residual genomic DNA, according to the protocols supplied with the RiboPure Bacterial Kit.

Full paper   Login or join for free to view the full paper.

Reviews

RiboPure™ RNA Purification Kit, bacteria from Thermo Fisher Scientific has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Discussion

2 years ago

Author: Paul G. Macon United States

Some help with RNA isolation using Trizol

Hello! I used Trizol to extract total RNA from in-vitro cultured bacteria (1 X 10^8 cells). After phase separation, I mixed ~0.4 ml of the upper phase which contains RNA with 0.5 mL cold isopropanol. However, the amount of RNA when measured in Nanodrop was very low. In addition, the ratio between 260 and 230 was around 0.1 to 0.5. Is there a chance that my sample was contaminated by the Trizol reagent? When I collected the aqueous phase I made sure to not touch the lower phase. What should I do?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Bacteria - Gram negative Salmonella enterica using RiboPure™ RNA Purification Kit, bacteria from Thermo Fisher Scientific.

Paper title
RNA Sequencing Reveals Differences between the Global Transcriptomes of Serovar Enteritidis Strains with High and Low Pathogenicities
Full paper
Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for RiboPure™ RNA Purification Kit, bacteria below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing RNA isolation / purification Bacteria - Gram negative Salmonella enterica using RiboPure™ RNA Purification Kit, bacteria from Thermo Fisher Scientific. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Become shareholder Discussions About us Contact Privacy Terms