EpiTect Bisulfite Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
For best results, the CT Conversion Reagent should be prepared freshly and used immediately following preparation.	Always include fully methylated (in-vitro methylated DNA) and non-methylated controls (Leucocyte DNA, DNMT1/3 double KO DNA) during MSP

Upstream tips
For best results, the CT Conversion Reagent should be prepared freshly and used immediately following preparation.
Protocol tips
Always include fully methylated (in-vitro methylated DNA) and non-methylated controls (Leucocyte DNA, DNMT1/3 double KO DNA) during MSP

Publication protocol

DNA extraction and pyrosequencing of blood, tumors and cell lines
DNA was extracted from 100 µl of blood or 25mg tumor tissue using DNeasy Blood & Tissue Kit (Qiagen). Sodium bisulfite conversion of genomic DNA, cleanup, and LINE1 and specific gene pyrosequencing analysis was performed by EpigenDx Inc. Primers are listed in Supplementary Table S2. For cell lines, genomic DNA extraction was performed using the QIAamp DNA extraction kit and 100 ng-2 µg of genomic DNA was converted to bisulfite DNA using the EpiTect® Plus DNA Bisulfite Kit (Qiagen). Pyrosequencing analysis of LINE1 elements, MLH1 and ZIC1 was performed using the PyroMark Q24 in conjunction with PyroMark Q24 CpG LINE1, CpG MLH1 and CpG ZIC1 assay kits (Qiagen).

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Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA methylation profiling Whole genome profiling - OVCAR-3 human ovarian cancer using EpiTect Bisulfite Kit from Qiagen.

Manufacturer protocol

Download the product protocol from Qiagen for EpiTect Bisulfite Kit below.

Download manufacturer protocol

Videos

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