CpGenome Universal DNA Modification Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
For best results, the CT Conversion Reagent should be prepared freshly and used immediately following preparation.

Upstream tips
For best results, the CT Conversion Reagent should be prepared freshly and used immediately following preparation.

Publication protocol

Bisulfite sequencing analysis
Genomic DNA was isolated from cells with QIAamp DNA kit (Qiagen). 1 µg of DNA was treated with the CpGenome™ DNA Modification kit (Millipore) according to the manufacturer’s manual. Treated DNA was dissolved in 25 µl H2O, and 1 µl of treated DNA was used in each PCR reaction as previously described (9). The following primers were used: H19 region 1: forward, 5’-TTGTAAGTGTGGATTTAAAAGT-3’, reverse, 5’-ACAATTATCAATTCAAA AAAAA-3’; H19 region 2: 5’-TTTTGGAGGTTTTTTTTTTA-3’, 5’-AAACCCTACAA CACCTAACT-3’; H19 region 3: 5’-GGTGGTAGGAAGGGGTTTTT-3’, 5’-CCCAACACCCA TCCTAAAAT-3’. The PCR products were subsequently cloned using the TOPO TA cloning kit (Life Technologies), and clones containing the converted DNA inserts were selected for sequencing. DNA sequences were analyzed with BiQ analyzer.

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Papers

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Manufacturer protocol

Download the product protocol from Millipore for CpGenome Universal DNA Modification Kit below.

Download manufacturer protocol

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