Xfect™ Transfection Reagent

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Always add your plasmid to the buffer before adding Xfect Polymer. dilute 5 µg of your plasmid DNA with Xfect Reaction Buffer to a final volume of 100 µl. Incubate for 10 min at room temperature. Add the nanoparticle complex solution dropwise to the cell culture medium. Incubate the plate at 37°C for 4 hr to overnight.

Protocol tips
Always add your plasmid to the buffer before adding Xfect Polymer. dilute 5 µg of your plasmid DNA with Xfect Reaction Buffer to a final volume of 100 µl. Incubate for 10 min at room temperature. Add the nanoparticle complex solution dropwise to the cell culture medium. Incubate the plate at 37°C for 4 hr to overnight.

Publication protocol

SIRT1 small interfering RNA (siRNA) was synthesized by Genepharma Corp. (Shanghai, China). The sequences of mouse SIRT1 siRNA were: 5’-UUG CAGAAACAGUGAGAAA-3’. The negative control siRNA was from Genepharma. Transfection of siRNA was performed by using the Xfect siRNA transfection reagents (Takara, Japan).

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Discussion

Papers

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Manufacturer protocol

Download the product protocol from Takara Bio Inc for Xfect™ Transfection Reagent below.

Download manufacturer protocol

Videos

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