EZchange™ Multi Site-directed Mutagenesis core Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
		For the mutagenesis and transformation control, spread cells on LB-ampicillin agar plate with 80 μg/ml X-gal and 20 mM IPTG

Downstream tips
For the mutagenesis and transformation control, spread cells on LB-ampicillin agar plate with 80 μg/ml X-gal and 20 mM IPTG

Publication protocol

Plasmids and site-directed mutagenesis
N-terminally GFP-tagged wild-type human FUS cDNA was cloned into the pReceiver vector (Genecopoeia). To make the mutant DNA (p.Q519E, p.G504Wfs*12, p.R495*), in vitro mutagenesis of the GFP-tagged FUS cDNA was conducted using the EZchange™ site-directed mutagenesis kit (Enzynomics) according to the manufacturer’s protocol.

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Discussion

Papers

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Manufacturer protocol

Download the product protocol from Enzynomics for EZchange™ Multi Site-directed Mutagenesis core Kit below.

Download manufacturer protocol

Videos

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