FACS CD14 Mouse

Get tips on using Mouse Adiponectin ELISA to perform ELISA Mouse - Adiponectin

Get tips on using Mouse Epidermal Growth Factor Receptor (EGFR) ELISA Kit to perform ELISA Mouse - EGFR

Get tips on using Mouse Von Willebrand Factor A2 ELISA Kit (ab208980) to perform ELISA Mouse - vWF-A2

Flow cytometry is an immunophenotyping technique whereby sing cell suspensions are stained for either cell surface markers or intracellular proteins by fluorescently-labelled antibodies and analyzed with a flow cytometer, where fluorescently-labelled molecules are excited by the laser to emit light at varying wavelengths, which is then detected by the instrument. There are several key criteria which are required to be kept in mind while designing a flow experiment- 1. Antibody titration (optimal dilution of antibodies should be calculated in order to avoid over- or under- saturated signals for proper detection of surface and intracellular markers), 2. Precision (3 or more replicates of the sample should be used per experiment), 3. Specificity (proper isotype controls should be included in the experiment), 4. Day-to-day variability (experiments should be repeated 3 or more times to ensure consistency and avoid variability due to flow cytometer settings), 5. Antibody interaction (Fluorescence minus one or FMO should be used, which is the comparison of signals from panel minus one antibody vs. the full panel), and 6. Antibody stability (fluorescently-labelled antibodies should be stored at 4C).

Get tips on using Mouse ANGPTL3 ELISA to perform ELISA Mouse - Angiopoietin-Like 3 (AngptL3)

Get tips on using PE-Cy™7 Mouse Anti-Human CD10 to perform Flow cytometry Anti-bodies Human - CD10

Get tips on using PE-Cy™7 Mouse Anti-Human CD117 to perform Flow cytometry Anti-bodies Human - CD117

Get tips on using PE-Cy™7 Mouse Anti-Human CD41a to perform Flow cytometry Anti-bodies Human - CD41