Protein transfection

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Get tips on using TransIT®-LT1 Transfection Reagent to perform siRNA / RNAi /miRNA transfection Human Cells - Cal 27 cells Polymer / lipid

Products Mirus TransIT®-LT1 Transfection Reagent

Get tips on using FuGENE® 6 Transfection Reagent to perform DNA transfection Mammalian cells - Primary cells Rat aortic smooth muscle cells (rASMC)

Products Promega FuGENE® 6 Transfection Reagent

Get tips on using Lipofectamine® 2000 Transfection Reagent to perform siRNA / RNAi /miRNA transfection Mouse - Lung adenocarcenoma (A549/LTEP-a-2) Lipofectamine

Products Thermo Fisher Scientific Lipofectamine® 2000 Transfection Reagent

Get tips on using Attractene Transfection Reagent to perform shRNA gene silencing Human - HEK 293T CAPN5- (Calpains) cationic lipid based

Products Qiagen Attractene Transfection Reagent

Get tips on using Lipofectamine® 2000 Transfection Reagent to perform DNA transfection Mammalian cells - Primary cells Rat pulmonary artery smooth muscle cell (pPASMC)

Products Thermo Fisher Scientific Lipofectamine® 2000 Transfection Reagent

Get tips on using FuGENE® 6 Transfection Reagent to perform DNA transfection Mammalian cells - Primary cells Rat pulmonary artery smooth muscle cell (pPASMC)

Products Promega FuGENE® 6 Transfection Reagent

Get tips on using FuGENE® HD Transfection Reagent to perform DNA transfection Mammalian cells - Primary cells Human pulmonary artery smooth muscle cells (HPASMC)

Products Promega FuGENE® HD Transfection Reagent

Get tips on using Lipofectamine® 2000 Transfection Reagent to perform DNA transfection Mammalian cells - Primary cells Human pulmonary artery smooth muscle cells (HPASMC)

Products Thermo Fisher Scientific Lipofectamine® 2000 Transfection Reagent

I would like to regulate the expression of a gene and in order to do that, I have purchased specific siRNA. After optimizing my transfection protocol and using electroporation I have achieved a 60-70% reduction of the gene of interest. However, I cannot observe a significant reduction of mRNA expression but only a reduction of protein. What might be the problem? Could the problem be in my cell treatment method?

Discussions siRNA/RNAi/miRNA transfection human

Get tips on using UltraCruz® Transfection Reagent to perform CRISPR Human - Activation GRP78

Products Santa Cruz Biotechnology UltraCruz® Transfection Reagent

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