Microarray Gene expression arrays Rat pancreas tissue

Get tips on using Expi293™ Expression System Kit to perform Protein expression and purification Mammalian cells - HEK 293 HER2 leader peptide

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Get tips on using Rat NGF/NGF Beta PicoKine™ ELISA Kit to perform ELISA Rat - beta-NGF

Get tips on using Rat Lipocalin-2/NGAL PicoKine™ ELISA Kit to perform ELISA Rat - NGAL/LCN2

Get tips on using Rat KIM-1 TIM-1 ELISA Kit (ab119597) to perform ELISA Rat - KIM-1

Get tips on using Rat heme oxygenase 1,HO-1 ELISA Kit to perform ELISA Rat - HO-1

Get tips on using Rat soluble E-selectin,sE-selectin ELISA Kit to perform ELISA Rat - sE-Selectin

Get tips on using Mouse/Rat Leptin Quantikine ELISA Kit to perform ELISA Mouse - Leptin

Get tips on using Active BDNF (Human, Rat) ELISA Kit to perform ELISA Mouse - GDNF