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|Add 100 µL/well (96-well plate) or 25 µL/well of CytoCalcein™
Green/Propidium Iodide dye-working solution.
Incubate the plate at room temperature or 37°C for 30 minutes to 1 hour, protected from light.
|Monitor the fluorescence intensity with a fluorescence plate reader (bottom read mode) at Ex/Em = 490/525 nm|