RNA isolation / purification Cells Cancer cell lines

Isolating DNA from tissues and paraffin-embedded tissue samples can be challenging as double-stranded DNA is physically fragile and highly susceptible to exo- and endonucleases. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in the presence of DNAse inhibitors. Further, extracting DNA from the nucleus need specific methods by combining physical, mechanical and chemical lysis approaches,

Get tips on using In Situ Cell Death Detection Kit, Fluorescein to perform TUNEL assay cell type - HeLa cells human cervical cancer

Get tips on using DNA Isolation Kit for Cells and Tissues to perform DNA isolation / purification Cells - Primary cells Human primary keratinocytes

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

Get tips on using TRIzol™ Max™ Bacterial RNA Isolation Kit to perform RNA isolation / purification Bacteria - Gram negative Vibro parahaemolyticus

Get tips on using Live/Dead Cell Double Staining Kit to perform Live / Dead assay mammalian cells - MCF-7 human breast cancer cells

Get tips on using Total Exosome RNA & Protein Isolation Kit to perform Protein isolation Mammalian cells - HeLa

Get tips on using GeneJET RNA Purification Kit to perform AAA for reviews

Get tips on using Gentra Puregene Buccal Cell Kit (100) to perform DNA isolation / purification Cells - Primary cells Buccal cells