Flowcytometry CD3 Mouse / IgG1, kappa Human

Get tips on using EasySep™ Human Cord Blood CD34 Positive Selection Kit II to perform Cell Isolation CD34+ cells

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Get tips on using Anti-phospho-Histone H2A.X (Ser139) Antibody to perform Immunohistochemistry γH2AX - Mouse Human -NA-

Flow cytometry is an immunophenotyping technique whereby sing cell suspensions are stained for either cell surface markers or intracellular proteins by fluorescently-labelled antibodies and analyzed with a flow cytometer, where fluorescently-labelled molecules are excited by the laser to emit light at varying wavelengths, which is then detected by the instrument. There are several key criteria which are required to be kept in mind while designing a flow experiment- 1. Antibody titration (optimal dilution of antibodies should be calculated in order to avoid over- or under- saturated signals for proper detection of surface and intracellular markers), 2. Precision (3 or more replicates of the sample should be used per experiment), 3. Specificity (proper isotype controls should be included in the experiment), 4. Day-to-day variability (experiments should be repeated 3 or more times to ensure consistency and avoid variability due to flow cytometer settings), 5. Antibody interaction (Fluorescence minus one or FMO should be used, which is the comparison of signals from panel minus one antibody vs. the full panel), and 6. Antibody stability (fluorescently-labelled antibodies should be stored at 4C).

Get tips on using PE anti-human CD114 (G-CSFR) Antibody to perform Flow cytometry Anti-bodies Human - CD114

Get tips on using CD11b Antibody, anti-human, PE, REAfinity™ to perform Flow cytometry Anti-bodies Human - CD11b

Get tips on using FITC anti-human CD15 (SSEA-1) Antibody to perform Flow cytometry Anti-bodies Human - CD15

Get tips on using Human ICAM-1/CD54 Antibody to perform Western blotting ICAM-1

Get tips on using ON-TARGETplus Human CDK1 (983) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - HEK293 CDK1