siRNA / miRNA gene silencing Human Primary Human Aortic Endothelial Cells

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Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human lung microvascular endothelial cells

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Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human dermal microvascular endothelial cells

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Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human coronary artery endothelial cells

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Get tips on using ZR RNA MiniPrepTM kit to perform RNA isolation / purification Cells - primary human endothelial cells

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Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Cellular assays Reporter gene assay luciferase HEK 293 human embryonic kidney cells

Get tips on using SurePrint Human miRNA Microarrays to perform Microarray Human - Endometrial stromal cells miRNA-expression array (labelled)

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Get tips on using X-tremeGENE™ HP DNA Transfection Reagent to perform DNA transfection Mammalian cells - Primary cells Human aortic smooth muscle cells (HOSMC)

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Get tips on using Stealth siRNA_SPI1 to perform siRNA / miRNA gene silencing Human - LAD2 PU.1/SPI1

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Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary human placental venous endothelial cells

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Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary human placental arterial endothelial cells

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