Protein expression and purification Yeast

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pHR-CMV-TetO2-VSV-G Product

Get tips on using pHR-CMV-TetO2-VSV-G to perform Protein Expression Eukaryotic cells - HEK293 VSV-G

Products Christian Siebold, Division of Structural Biology, Wellcome Cent pHR-CMV-TetO2-VSV-G

ELISA Human - Chitinase-3-Like Protein-1 (CHI3L1) or YKL-40 Experiment

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

Proteins ELISA Human Chitinase-3-Like Protein-1 (CHI3L1) or YKL-40

ELISA Mouse - Chitinase-3-Like Protein-1 (CHI3L1) or YKL-40 Experiment

Proteins ELISA Mouse Chitinase-3-Like Protein-1 (CHI3L1) or YKL-40

Qproteome Bacterial Protein Prep Kit Product

Get tips on using Qproteome Bacterial Protein Prep Kit to perform Protein isolation Bacteria - Bacillus subtilis