rna-isolation-purification-cells-primary-mouse-dorsal-root-ganglion-neurons

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N788 phenol-free total RNA purification kit Product

Get tips on using N788 phenol-free total RNA purification kit to perform RNA isolation / purification Bacteria - Gram negative Pseudomonas aeruginosa

Products Amresco N788 phenol-free total RNA purification kit

RNAsimple Total RNA Kit Product

Get tips on using RNAsimple Total RNA Kit to perform RNA isolation / purification Cells - immortalized MA-104

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Protein isolation Mammalian cells - Mouse_Brown fat Experiment

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.

Proteins Protein isolation Mammalian cells Mouse_Brown fat

XcelGen Fungal RNA Isolation Mini Kit Product

Get tips on using XcelGen Fungal RNA Isolation Mini Kit to perform RNA isolation / purification Fungal

Products Xcelris Genomics XcelGen Fungal RNA Isolation Mini Kit

PicoPure™ RNA Isolation Kit Product

Get tips on using PicoPure™ RNA Isolation Kit to perform RNA isolation / purification Tissue - human brain tissue

Products Thermo Fisher Scientific PicoPure™ RNA Isolation Kit

SV Total RNA Isolation System Product

Get tips on using SV Total RNA Isolation System to perform RNA isolation / purification Tissue - human liver tissue

Products Promega SV Total RNA Isolation System

SV Total RNA Isolation System Product

Get tips on using SV Total RNA Isolation System to perform RNA isolation / purification Bacteria - Gram positive Staphylococcus saprophycitius

Products Promega SV Total RNA Isolation System

High Pure RNA Isolation Kit Product

Get tips on using High Pure RNA Isolation Kit to perform RNA isolation / purification Bacteria - Gram positive Staphylococcus saprophycitius

Products Roche Lifesciences High Pure RNA Isolation Kit

High Pure RNA Isolation Kit Product

Get tips on using High Pure RNA Isolation Kit to perform RNA isolation / purification Bacteria - Gram positive Lactobacillus amylovorus

Products Roche Lifesciences High Pure RNA Isolation Kit

High Pure RNA Isolation Kit Product

Get tips on using High Pure RNA Isolation Kit to perform RNA isolation / purification Bacteria - Gram negative Legionella pneumophilia

Products Roche Lifesciences High Pure RNA Isolation Kit

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