Cell Culture Contamination Detection Kit

Get tips on using RPMI-1640 Medium to perform Mammalian cell culture media U937

Get tips on using DULBECCO MEM MEDIUM to perform Mammalian cell culture media U87

Get tips on using DULBECCO MEM MEDIUM to perform Mammalian cell culture media T98G

Get tips on using DMEM/F-12 to perform Mammalian cell culture media T47D

Get tips on using RPMI-1640 Medium to perform Mammalian cell culture media PNT1A

Get tips on using DMEM/F-12 to perform Mammalian cell culture media A2780

Get tips on using CD CHO Medium to perform Mammalian cell culture media CHO

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

DNA damage assay is a standard method for determining in-vivo/in-vitro genotoxicity by measuring the breaks in the DNA chain of animal and plant cells. Initial DNA damage leads to cell cycle arrest and, at the final stages, leads to induction of senescence or cell death (apoptosis, necrosis, autophagy, or mitotic catastrophe). Detection of DNA damage from mild to moderate to severe is challenging when studying genotoxicity in the pool of cells. It is favorable to use DNA damage assay kits available for prominent identification of the extent of damage in the analysis.

Get tips on using Advanced DMEM to perform 3D Cell Culture Media Murina esophageal organoids