Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Cells - immortalized OS-RC-2
Get tips on using TRI Reagent® Sigma to perform RNA isolation / purification Cells - immortalized Mono-Mac-6
Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Cells - immortalized HeLa, CaSki and C33A (Cervical cancer cells)
Get tips on using FastLane Cell Probe Kit (200) to perform RNA isolation / purification Cells - immortalized HT-29
Get tips on using Wizard® Genomic DNA Purification Kit to perform DNA isolation / purification Cells - Immortalized cell lines 3T3
Get tips on using Cell DNA Isolation Kit to perform DNA isolation / purification Cells - Immortalized cell lines C2C12
Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Cells - immortalized SKBR3, MDA-MB231 and MCF7
Get tips on using FastLane Cell Probe Kit (200) to perform RNA isolation / purification Cells - immortalized MIA PaCa-2
RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.
Get tips on using Wizard® Genomic DNA Purification Kit to perform DNA isolation / purification Cells - Immortalized cell lines HeLa
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